Anti-C-Peptide Rabbit Monoclonal Antibody for IF/ICC, IF-P, IHC-P, ELISA - P01308

Core Product Specifications and Parameters

Biological Background: C-Peptide and Insulin Function and Localization

• Insulin is a key hormone that decreases blood glucose concentration by enhancing cellular permeability to monosaccharides, amino acids, and fatty acids, and by accelerating glycolysis, the pentose phosphate cycle, and glycogen synthesis in the liver.
• The insulin gene encodes preproinsulin, which is processed into proinsulin and subsequently cleaved into the B-chain, C-peptide, and A-chain; C-peptide is co-secreted with mature insulin in equimolar amounts.
• C-peptide, historically considered a byproduct of insulin maturation, is now recognized for potential physiological roles and serves as a clinically important biomarker of endogenous insulin secretion.
• The protein is expressed and secreted by pancreatic beta-cells, with tissue-specific detection at the protein level in these cells.
• UniProt keywords highlight its involvement in carbohydrate metabolism, glucose metabolism, and its association with diabetes mellitus; the protein is a secreted hormone with a signal peptide and multiple disulfide bonds.
• This antibody was generated against a synthetic peptide spanning amino acids 1-100 of insulin, covering the B-chain and a major portion of C-peptide, making it suitable for detecting C-peptide and proinsulin forms.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 1-100 of human INS) encompasses the B-chain and most of C-peptide; therefore, the antibody may recognize proinsulin and partially processed intermediates, but not mature insulin (which lacks the C-peptide region). For specific C-peptide detection, validate against proinsulin cross-reactivity using appropriate controls.
• For ELISA applications, consider using the antibody in a capture or detection format after confirming linearity and specificity with recombinant C-peptide and proinsulin standards in your sample matrix.
• In immunohistochemistry (IHC-P), paraffin-embedded pancreatic tissue sections should show strong staining in islet beta-cells; antigen retrieval may be required for optimal signal.
• For immunofluorescence (IF/ICC and IF-P), permeabilization is recommended to access intracellular C-peptide stores; include an isotype control to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to deliver a comprehensive portfolio of validated raw materials for diabetes and metabolic disease biomarker development. Supporting assays for C-peptide—a critical marker of beta-cell function—we offer ready-to-use matched antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target needs. From early feasibility through clinical validation, CamelBio streamlines your supply chain so you can focus on assay performance.