Anti-CAMP Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P49913

Core Product Specifications and Parameters

Biological Background: CAMP Function and Localization

• CAMP encodes the human cathelicidin antimicrobial peptide (hCAP-18), which is proteolytically processed to generate the mature LL-37 peptide and other antimicrobial fragments (PubMed:14978112, PubMed:9736536). Related references: PMID:14978112 PMID:9736536
• LL-37 is an integral component of innate immunity, binding bacterial lipopolysaccharides (LPS) and disrupting microbial membranes via pore formation (PubMed:16637646, PubMed:18818205, PubMed:22879591, PubMed:32753597, PubMed:33060695, PubMed:10417311). Related references: PMID:16637646 PMID:18818205 PMID:22879591 PMID:32753597 PMID:33060695 PMID:10417311
• The peptide exhibits broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant S. aureus (MRSA) and vancomycin-resistant enterococci (PubMed:10417311, PubMed:32753597, PubMed:8681941, PubMed:9736536). Related references: PMID:10417311 PMID:32753597 PMID:8681941 PMID:9736536
• LL-37 displays synergistic bactericidal effects with other cathelicidin fragments (e.g., KS-30, KR-31) and maintains activity under elevated salt conditions (PubMed:14978112). Related references: PMID:14978112
• Beyond direct killing, LL-37 modulates host immune responses by inducing cytokines and chemokines in keratinocytes and acting via neutrophil receptors to enhance CXCL2 release (PubMed:15778390, PubMed:22879591). Related references: PMID:15778390 PMID:22879591
• CAMP is highly expressed in neutrophilic granulocytes and bone marrow, and is also secreted into sweat for topical skin defense (PubMed:7529412, PubMed:7615076, PubMed:7890387, PubMed:8681941, PubMed:8946956, PubMed:9736536, PubMed:14978112). Related references: PMID:7529412 PMID:7615076 PMID:7890387 PMID:8681941 PMID:8946956 PMID:9736536 PMID:14978112
• Subcellularly, the protein is secreted and found in vesicles, consistent with its role in extracellular microbial killing.
• The precursor protein has a molecular weight of ~19 kDa and features a signal peptide, a cathelin domain, and the antimicrobial LL-37 domain, with processing at dibasic sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 71–170 of human CAMP, covering the LL-37 domain; the antibody may recognize both the full-length precursor and processed mature peptide.
• For WB under reducing conditions, expect a band near 19 kDa (precursor) and possibly lower molecular weight bands corresponding to processed forms. Validate in relevant sample systems.
• In IHC-P, consider using tissues known to express CAMP, such as bone marrow or skin biopsies; adequate permeabilization may be required for intracellular detection.
• For ELISA, pairing with a suitable detection antibody may enhance sensitivity for the mature LL-37 peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by offering a comprehensive portfolio of high-performance antibodies and reagents for infectious disease and innate immunity research. From validated anti-CAMP monoclonal antibodies to matched detection pairs and bulk ancillary materials, we provide reliable solutions to support diagnostic assay development. Whether you require routine sourcing or access to rare antimicrobial peptide targets, CamelBio serves as your single partner from concept to clinic.