Anti-SFTPC Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P11686

Core Product Specifications and Parameters

Biological Background: SFTPC Function and Localization

• SFTPC encodes surfactant protein C (SP-C), a highly hydrophobic pulmonary surfactant-associated protein, also known as SP5, PSP-C, or SFTP2.
• SP-C is an essential component of pulmonary surfactant, reducing surface tension at the alveolar air-liquid interface to prevent lung collapse and facilitate gas exchange.
• The protein is synthesized as a 21 kDa proprotein (UniProt molecular weight 21,013 Da) and undergoes proteolytic processing and palmitoylation to generate the mature active form.
• SFTPC is classified as a secreted lipoprotein and contains disulfide bonds, as highlighted by UniProt keywords (Lipoprotein, Palmitate, Disulfide bond).
• SP-C is primarily localized in the extracellular surfactant film lining the alveoli, consistent with its role in respiratory function.
• Alternative splicing of the SFTPC gene may generate multiple isoforms, although the functional significance in surfactant composition is not fully characterized.
• Mutations in SFTPC are associated with interstitial lung diseases and other respiratory pathologies, underscoring its clinical relevance in pulmonary disorders.

Experimental Guidance and Technical Tips

• The immunogen used was recombinant human SFTPC protein; therefore, the antibody is expected to recognize denatured and native forms in Western blotting (WB), immunofluorescence (IF-P), and ELISA.
• For WB, a band near 21 kDa is anticipated under reducing conditions; however, due to the hydrophobic nature of SP-C, aggregation artifacts or higher molecular weight bands may occur. Consider optimizing sample preparation (e.g., heating at lower temperatures, using non-reducing conditions for certain applications).
• In IF-P, SP-C localizes to lamellar bodies and the extracellular surfactant layer in alveolar type II cells. Fixation with paraformaldehyde and permeabilization with mild detergents is recommended to detect this secreted protein.
• Cross-reactivity with mouse and rat SFTPC has been confirmed, making this antibody suitable for translational studies and preclinical models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering validated monoclonal and polyclonal antibodies, recombinant proteins, and bulk ancillary reagents. The anti-SFTPC rabbit monoclonal antibody is available through our one-stop platform, addressing research and development needs in respiratory disease diagnostics. Leverage our expertise to source rare targets, optimize assay performance, and accelerate your IVD pipeline from concept to clinic.