Anti-PRD Rabbit Monoclonal Antibody for WB, ELISA - P12955

Core Product Specifications and Parameters

Biological Background: PRD Function and Localization

• PRD (PEPD) is a member of the M24 metallopeptidase family, also known as Xaa-Pro dipeptidase, imidodipeptidase, proline dipeptidase, or prolidase.
• It catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue at the C-terminus, with Gly-Pro being the preferred substrate. Related references: PMID:17081196, PMID:35165443
• This enzyme plays a critical role in collagen metabolism by recycling proline from collagen degradation products. Related references: PMID:2925654
• PRD requires manganese (Mn²⁺) as a cofactor for its catalytic activity and is classified as a manganese-dependent metalloprotease.
• The PEPD gene undergoes alternative splicing, producing multiple transcript variants. Post-translational modifications include acetylation and phosphorylation.
• Mutations in PEPD are associated with prolidase deficiency, a rare autosomal recessive disorder characterized by impaired collagen turnover (disease variant).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 400-493) of human PRD, which may provide target specificity; consider validating the antibody in your sample system.
• For Western blotting, a specific band around 55 kDa is expected. Optimize sample loading amounts and antibody dilution for your experimental conditions.
• In ELISA applications, use a properly validated coating buffer and detection system to ensure reliable results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. This PRD antibody supports research into collagen metabolism and prolidase-related disorders, aligning with our commitment to supply high-quality IVD raw materials for every stage of development. Partner with CamelBio to streamline your sourcing and accelerate your projects from concept to clinic.