Anti-Siglec-8 Rabbit Monoclonal Antibody for WB, FC, ELISA - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Encoded by the SIGLEC8 gene, Siglec-8 (also known as SAF-2, SIGLEC8L) is a member of the sialic acid-binding immunoglobulin-like lectin family and undergoes alternative splicing.
• Functions as a putative adhesion molecule that mediates sialic acid-dependent binding to blood cells, preferentially recognizing α-2,3- and α-2,6-linked sialic acids, with the recognition site potentially masked by cis interactions on the same cell surface. (PubMed:10625619, PubMed:10856141) Related references: PMID:10625619 PMID:10856141
• Exhibits high specificity for terminal N-acetylneuraminic acid with an underlying 6-O-sulfated galactose, showing a strong selectivity for Gal-6-sulfated sialyl-Lewis X glycan structures. (PubMed:27357658) Related references: PMID:27357658
• Localized to the cell membrane, consistent with its role in extracellular adhesion and signaling.
• Tissue expression is restricted to blood cells, specifically on eosinophils, basophils, and mast cells, implicating it in allergic and inflammatory responses.
• Features include a signal peptide, Ig-like domains, transmembrane helix, disulfide bonds, and glycosylation motifs, marking it as a structurally complex immune receptor.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 17–363), corresponding to the mature protein without the signal peptide; verify detection in native versus recombinant systems.
• For Western blotting, expect a band around 54 kDa in lysates from eosinophil, basophil, or transfected cell lines expressing Siglec-8.
• In flow cytometry on live cells, consider that cis-bound sialic acids may mask the epitope; evaluate sialidase treatment or optimized blocking steps.
• Cross-reactivity with mouse and rat indicates a conserved epitope; validate the antibody in your specific species and sample type.
• ELISA applications may require optimization of coating antigen and detection conditions due to the lectin’s glycan-binding properties.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless, end-to-end sourcing solution for IVD raw materials, accelerating your development from concept to clinic. For Siglec-8-focused research in allergy, asthma, and immune regulation, CamelBio supplies validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, alongside expert support for rare-target raw material sourcing. Partner with CamelBio to streamline your diagnostic assay development.