Anti-p53 Rabbit Monoclonal Antibody for WB, IP, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53, also known as Cellular tumor antigen p53, Phosphoprotein p53, and Tumor suppressor p53, is encoded by the TP53 gene. Multiple isoforms exist (e.g., isoform 2 enhances transactivation activity, isoform 4 suppresses transactivation, isoform 7 inhibits apoptosis) with distinct functional properties.
• Acts as a multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence, functioning as a tumor suppressor in many tumor types.
  Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Apoptosis induction is mediated partly by stimulation of BAX and FAS antigen expression, or by repression of Bcl‑2 expression; its pro‑apoptotic activity is regulated via interactions with ASPP family proteins.
  Related references: PMID:12524540, PMID:17189187
• Subcellular localization includes the nucleus, cytoplasm, mitochondria, endoplasmic reticulum, and centrosome, allowing dynamic shuttling in response to cellular stress signals.
• Undergoes extensive post‑translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, which modulate its stability and transcriptional activity.
• Regulates the circadian clock by repressing CLOCK‑BMAL1‑mediated transcriptional activation of PER2.
  Related references: PMID:24051492
• Ubiquitously expressed across normal tissues with isoform‑dependent patterns; for example, isoform 2 is absent in brain, lung, and prostate, while isoform 8 is detected only in colon, bone marrow, testis, fetal brain, and intestine.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein covering amino acids 1–393 of human p53 (NP_000537.3), encompassing nearly the full‑length sequence; this design may confer recognition of epitopes across multiple functional domains, suitable for applications such as Western blotting, immunoprecipitation, and ELISA.
• As a KO‑validated monoclonal antibody, it is expected to exhibit high specificity; consider using a p53‑knockout cell lysate as a negative control in Western blotting to confirm target band identity.
• For Western blotting, the predicted molecular weight is 44 kDa, but post‑translational modifications and isoform variations may result in multiple bands or a slight shift; optimize loading amounts and exposure times accordingly.
• For immunoprecipitation, use standardized protocols and include an isotype‑control antibody to assess non‑specific binding.
• In ELISA, pair this antibody with an appropriate detection antibody following standard procedures; validate in the relevant sample matrix to ensure performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. With expertise in p53 and tumor‑suppressor‑related targets, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized rare‑target material sourcing to accelerate your assay development.