Anti-APOL1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O14791

Core Product Specifications and Parameters

Biological Background: APOL1 Function and Localization

• APOL1 encodes apolipoprotein L1, a secreted glycoprotein and member of the apolipoprotein L family; alternatively spliced isoforms exist.
• It may play a role in lipid exchange and transport, potentially participating in reverse cholesterol transport from peripheral cells to the liver.
• APOL1 is a crucial component of trypanosome lytic factor in human serum; it forms pores in parasite lysosomal membranes and sensitizes Trypanosoma brucei to osmotic lysis. Related references: PMID:12621437 PMID:16020735 PMID:19997494
• It is secreted and found in plasma, primarily associated with APOA-I-containing high-density lipoprotein (HDL3).
• APOL1 is expressed in several tissues including pancreas, lung, prostate, liver, placenta, and spleen.
• Post-translational modifications include glycosylation and phosphorylation. It is associated with cholesterol metabolism, steroid metabolism, and lipid transport. Genetic variants are linked to disease.

Experimental Guidance and Technical Tips

• For Western blot, consider using reducing SDS-PAGE and loading appropriate positive control (e.g., human liver or plasma samples); expect a band around 44 kDa.
• For immunofluorescence/ICC, include a permeabilization step to detect intracellular pools; secretion pathway localization may require co-staining with Golgi markers.
• For ELISA, validate pairing and epitope availability with your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio meets your sourcing needs for high-quality APOL1 antibodies and ancillary reagents, helping accelerate your lipid metabolism or infectious disease diagnostic development. We offer validated antibody pairs, optimized monoclonals, and rare target sourcing to support every stage from concept to clinical application.