Anti-PABPN1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q86U42

Core Product Specifications and Parameters

Biological Background: PABPN1 Function and Localization

• PABPN1, also known as Polyadenylate-binding protein 2, Nuclear poly(A)-binding protein 1, or Poly(A)-binding protein II, is a ubiquitously expressed RNA-binding protein.
• It plays a key role in the 3'-end formation of mRNA precursors by stimulating poly(A) polymerase (PAPOLA) to add a poly(A) tail of 200–250 nucleotides, increasing polymerase processivity and controlling tail length.
• PABPN1 is involved in multiple stages of mRNA metabolism, including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD).
• It cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1, potentially regulating muscle-specific gene expression. Related references: PMID:11371506
• As a subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, PABPN1 directs a subset of long and polyadenylated RNAs for exosomal degradation. Related references: PMID:27871484
• The protein binds poly(A) and poly(G) with high affinity and may protect the poly(A) tail from degradation.
• Subcellular localization includes the nucleus, nuclear speckles, and cytoplasm.
• Post-translational modifications include acetylation, methylation, and phosphorylation; mutations in PABPN1, such as a GCG trinucleotide repeat expansion, are associated with oculopharyngeal muscular dystrophy (OPMD).

Experimental Guidance and Technical Tips

• As a rabbit monoclonal antibody, it offers high specificity and low background; consider validating in your specific assay system.
• The immunogen corresponds to the N-terminal 1–100 amino acids of human PABPN1; thus, the antibody may recognize the N-terminal region.
• For Western blot, the predicted molecular weight is around 31–33 kDa; note that the endogenous protein may exhibit a slightly different mobility due to post-translational modifications.
• In immunofluorescence (IF/ICC) and IF-P, expect nuclear and nuclear speckle staining patterns; cytoplasmic signal may also be observed.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using positive control tissues known to express PABPN1 (e.g., skeletal muscle).
• In ELISA applications, the antibody can be used as a capture or detection reagent; pair with an appropriate matched antibody for sandwich ELISA development.

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