Anti-PDCD4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q53EL6

Core Product Specifications and Parameters

Biological Background: PDCD4 Function and Localization

• Programmed cell death protein 4 (PDCD4) is a tumor suppressor that inhibits translation initiation and cap-dependent translation by interfering with the interaction between EIF4A1 and EIF4G, and by suppressing the helicase activity of EIF4A.
• PDCD4 modulates the activation of JUN kinase and down-regulates the expression of MAP4K1, thereby inhibiting MAPK85 activation and JUN-dependent transcription, which are critical for driving invasion.
• The protein may play a role in apoptosis and binds RNA, contributing to its regulatory functions in cell proliferation and survival.
• PDCD4 is predominantly localized in the nucleus and cytoplasm, allowing it to influence both transcriptional and translational machinery.
• Expression is up-regulated in proliferative cells and highly expressed in epithelial cells of the mammary gland, while reduced expression has been observed in lung cancer and colon carcinoma, consistent with its role as a tumor suppressor.
• The protein undergoes various post-translational modifications, including acetylation and phosphorylation, and is part of the ubiquitin conjugation pathway, reflecting complex regulatory control.
• Alternative splicing generates multiple isoforms, adding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 370-469 of human PDCD4; for Western blot (WB), a band around 52 kDa is expected in lysates from human, mouse, or rat samples.
• For immunohistochemistry (IHC-P), antigen retrieval optimization may be required; consider testing multiple fixation and retrieval conditions to achieve optimal staining.
• In ELISA, validate specificity using recombinant PDCD4 protein or peptide blocking assays; incubate primary antibody at appropriate dilutions based on preliminary checkerboard titrations.
• Due to the monoclonal nature, expect high target specificity; however, cross-reactivity with other isoforms or related proteins should be ruled out using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one-stop sourcing experience for IVD raw materials, including validated monoclonal antibodies like this anti-PDCD4 Rabbit mAb, which targets a key tumor suppressor in oncology research. Beyond antibodies, we supply optimized bulk ancillary reagents, antibody pairs for custom assay development, and rare-target raw materials, all backed by comprehensive technical support to accelerate your diagnostic projects from concept to clinic.