Anti-CtBP1 Monoclonal Antibody for WB, ELISA - Q13363

Core Product Specifications and Parameters

Biological Background: CtBP1 Function and Localization

• CtBP1 acts as a transcriptional corepressor, interacting with diverse transcription regulators including GLIS2 and BCL6.
• Possesses intrinsic dehydrogenase activity, suggesting a role in metabolic sensing or redox regulation.
• Involved in maintaining the balance between tubular and stacked structures of the Golgi complex.
• Functions in brown adipose tissue (BAT) differentiation, highlighting a potential role in energy metabolism.
• Encoded by the CTBP1 gene, the protein localizes to both the cytoplasm and nucleus, shuttling in response to cellular signals.
• Expressed in germinal center B-cells, indicating a specialized role in adaptive immunity.
• Subject to various post-translational modifications including ADP-ribosylation and phosphorylation, which modulate its function.
• Alternatively spliced transcript variants exist, potentially giving rise to isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 301-440 of human CtBP1, a region that may confer specificity to the C-terminal domain. Consider this when assessing cross-reactivity with CtBP2 or other family members.
• For Western blotting, the predicted molecular weight is 48 kDa, but endogenous protein may exhibit shifts due to post-translational modifications. Validate in the relevant sample system.
• For ELISA applications, the recombinant immunogen can serve as a positive control to confirm antibody binding. Optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities. For researchers studying transcriptional regulation, metabolic signaling, or Golgi dynamics, we provide comprehensive support to accelerate your diagnostic development from concept to clinic.