Anti-Human IgG Rabbit Monoclonal Antibody for WB, ELISA - P01857 / P01859 / P01860 / P01861

Core Product Specifications and Parameters

Biological Background: Human IgG (Immunoglobulin Heavy Constant Gamma Chains) Function and Localization

• Immunoglobulin heavy constant gamma chains are the constant regions of IgG antibodies, which are secreted or membrane-bound glycoproteins produced by B lymphocytes and central to adaptive humoral immunity. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• The antigen-binding specificity of antibodies is generated through V(D)J recombination, somatic hypermutations, and affinity maturation, resulting in high-affinity recognition of diverse pathogens. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• The constant region defines the immunoglobulin isotype; IgG1 heavy chain mediates effector functions via its Fc region, including antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and complement activation. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Upon B cell activation, class-switch recombination replaces the IgM/IgD constant region with IgG, IgA, or IgE, tailoring the immune response. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• The human IgG heavy chain constant region is encoded by four genes—IGHG1 (P01857), IGHG2 (P01859), IGHG3 (P01860), IGHG4 (P01861)—giving rise to the IgG1, IgG2, IgG3, and IgG4 subclasses with distinct effector profiles.
• IgG heavy chains are expressed at the B cell surface as part of the B cell receptor complex and are secreted into extracellular fluids upon plasma cell differentiation.
• Key post-translational modifications include N-linked glycosylation and intra- and inter-chain disulfide bonds, essential for structural stability and Fc-mediated effector interactions.

Experimental Guidance and Technical Tips

• For Western blotting, use a reducing sample buffer to dissociate inter-chain disulfide bonds; the heavy chain band is expected at ~52 kDa, while the light chain (~25 kDa) may not be detected if the antibody is heavy-chain specific.
• In ELISA applications, this rabbit monoclonal antibody can serve as either a capture or detection reagent for quantifying human IgG; optimal coating conditions and conjugate dilutions should be empirically determined.
• If cross-reactivity to individual IgG subclasses is critical, verify specificity using purified IgG1, IgG2, IgG3, and IgG4 proteins in your assay system.
• Avoid repeated freeze-thaw cycles; aliquot and store at -20°C or as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, offering validated antibody pairs, bulk monoclonal reagents, and ancillary materials for human IgG immunoassay development. Our platform supports manufacturers and researchers from early-stage concept to clinical application, with expertise in rare-target sourcing and scalable supply. Explore our IgG detection portfolio for diagnostic kit development and humoral immunity research.