Anti-C-Reactive Protein (CRP) Monoclonal Antibody for WB, IHC-P, ELISA - P02741

Core Product Specifications and Parameters

Biological Background: C-Reactive Protein (CRP) Function and Localization

• C-reactive protein (CRP) is an acute-phase protein encoded by the CRP (PTX1) gene in humans.
• It plays a key role in host defense by promoting agglutination, bacterial capsular swelling, phagocytosis, and complement fixation in a calcium-dependent manner via binding to phosphorylcholine.
• CRP can interact with DNA and histones, potentially scavenging nuclear material released from damaged circulating cells.
• The protein is secreted and found in plasma, consistent with its role as a systemic acute-phase reactant.
• Post-translational modifications include pyrrolidone carboxylic acid formation, a disulfide bond, and a signal peptide; the mature protein has a calculated molecular weight of approximately 25 kDa.
• UniProt keywords: acute phase, calcium, metal-binding, secreted, and direct protein sequencing.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is around 23-25 kDa; consider optimizing sample preparation to detect endogenous CRP, which may be low in healthy plasma but elevated during acute phase responses.
• In IHC-P, consider using appropriate positive control tissues known to have high CRP expression during inflammation (e.g., liver tissue from acute phase models) and validate antibody specificity with blocking peptide or isotype controls.
• For ELISA, pair this antibody with a suitable detection antibody; ensure appropriate antigen coating and validation in the relevant sample matrix (e.g., serum or plasma).
• The immunogen sequence spans a region conserved between human and mouse CRP, supporting the reported cross-reactivity; however, users should verify performance in their specific assays.
• Since CRP binds calcium, consider the effect of calcium concentration in buffers on antibody-antigen interaction during experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CRP-related inflammation and immune defense studies, we offer reliable monoclonal antibodies like this CRP (P02741) rabbit mAb, along with complementary antigens and technical support. Our end-to-end sourcing services help streamline your assay development from selection to validation.