Anti-CENPC Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q03188

Core Product Specifications and Parameters

Biological Background: Centromere protein C Function and Localization

• Centromere protein C (CENP-C), also known as CENPC1 or interphase centromere complex protein 7, is a key component of the centromere/kinetochore region essential for proper chromosome segregation during mitosis.
• CENP-C is a member of the CENPA-NAC (nucleosome-associated) complex, which is critical for the assembly of kinetochore proteins, mitotic progression, and faithful chromosome segregation.
• The CENPA-NAC complex facilitates the recruitment of the CENPA-CAD (nucleosome distal) complex and is implicated in the incorporation of newly synthesized CENPA into centromeres.
• CENP-C additionally plays a role in epigenetic regulation by recruiting DNA methyltransferase DNMT3B to centromeric and pericentromeric satellite repeats, thereby modulating the histone code in these regions.
• Subcellular localization is predominantly to the nucleus, specifically at the centromere and kinetochore structures of chromosomes.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond, Ubl conjugation), which may regulate its function during cell division.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 380–520 of human CENPC, a region expected to generate reactivity against the full-length protein.
• For western blotting (WB), a major band at approximately 107 kDa is anticipated in human and rat cell or tissue lysates; additional bands may appear due to post-translational modifications or isoforms.
• In immunofluorescence/immunocytochemistry (IF/ICC), a distinct punctate nuclear pattern consistent with centromere/kinetochore localization should be observed.
• ELISA performance has been validated by the supplier; users should optimize coating conditions and antibody dilutions for their specific assay setup.
• Validation in the intended sample system (e.g., specific human or rat cell lines) is recommended to confirm reactivity and minimal non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined one-stop platform for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CENPC monoclonal antibody supports studies in centromere biology, kinetochore assembly, chromosome dynamics, and mitotic regulation. Leverage CamelBio’s supply capabilities for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing to accelerate your IVD development and research projects.