Anti-CPE Rabbit Polyclonal Antibody for WB and ELISA - P16870

Core Product Specifications and Parameters

Biological Background: Carboxypeptidase E Function and Localization

• Carboxypeptidase E (CPE), also designated carboxypeptidase H (CPH), enkephalin convertase, and prohormone-processing carboxypeptidase, belongs to the metallocarboxypeptidase family.
• It operates as a sorting receptor, guiding prohormones to the regulated secretory pathway in neuroendocrine cells.
• As a processing enzyme, it removes C‑terminal dibasic residues from peptide hormone precursors after initial endoprotease cleavage.
• The protein is found in secretory vesicle membranes and is also secreted into the extracellular space.
• CPE is a zinc‑dependent metalloprotease that undergoes glycosylation; alternative splicing yields multiple isoforms.
• Mutations in the CPE gene are linked to obesity, hypogonadotropic hypogonadism, and intellectual disability, underscoring its critical endocrine role.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 257–476 of human CPE, a region likely to recognize both the full‑length and certain splice variants.
• For Western blot, the predicted molecular weight is ~53 kDa; consider optimizing protein extraction and transfer conditions for your specific sample type.
• In ELISA applications, coat with the appropriate antigen and validate using a standard curve to ensure sensitivity.
• The antibody’s cross‑reactivity with mouse makes it a candidate for parallel studies in mouse tissues or cell lines.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw‑material procurement, offering access to validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. This CPE polyclonal antibody supports research into prohormone processing and neuroendocrine regulation. Whether you require custom development or off‑the‑shelf reagents, CamelBio bridges your concept to clinic with reliable supply solutions.