Anti-PMPCA Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, IP, ELISA - Q10713

Core Product Specifications and Parameters

Biological Background: PMPCA Function and Localization

• PMPCA encodes mitochondrial-processing peptidase subunit alpha (Alpha-MPP), also known as P-55, an inactive zinc metalloprotease.
• It is the substrate-recognition subunit of the essential mitochondrial processing protease (MPP), which cleaves mitochondrial targeting sequences from precursor proteins imported into the organelle.
• Together with the beta subunit (PMPCB), PMPCA ensures proper maturation of nuclear-encoded mitochondrial proteins, critical for mitochondrial function.
• The protein localizes to the mitochondrial matrix and inner membrane, consistent with its role in processing precursors at the import site.
• PMPCA is ubiquitously expressed, with highest levels in fetal tissues and adult brain, particularly the cerebellum and cerebellar vermis, highlighting its importance in neurodevelopment.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms, potentially modulating activity.
• Mutations in PMPCA are linked to human diseases such as intellectual disability and neurodegeneration, as noted in UniProt keywords, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 291-525 of human PMPCA, covering the C-terminal region; this design may recognize both full-length and processed forms. Consider validating detection in mitochondrial-enriched fractions.
• In Western blot, the primary band is expected near 58 kDa. Minor shifts due to acetylation or isoform variations might occur; use reducing conditions for consistent results.
• Cross-reactivity with mouse and rat allows translational studies, but confirm sequence homology in your specific model system to avoid signal discrepancies.
• For immunoprecipitation and ELISA, the rabbit monoclonal format provides high affinity and lot-to-lot consistency; titrate the antibody to optimize for your sample type and assay conditions.
• In immunofluorescence and immunohistochemistry, mitochondrial staining can be confirmed by co-localization with a mitochondrial marker (e.g., TOM20). Antigen retrieval may be necessary for IHC-P.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including this anti-PMPCA rabbit monoclonal antibody for mitochondrial and neurological disease research. Our comprehensive portfolio features validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing solutions. With end-to-end support from concept to clinic, CamelBio is your trusted partner for accelerating assay development.