Anti-Haptoglobin (HP) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P00738

Core Product Specifications and Parameters

Biological Background: Haptoglobin (HP) Function and Localization

• Haptoglobin, also known as zonulin, is a secreted acute-phase glycoprotein predominantly produced by the liver.
• Captures free hemoglobin released during hemolysis, forming complexes that are cleared by CD163 on liver Kupffer cells, thereby recycling heme iron and preventing kidney damage.
• Exhibits antioxidant activity and has antibacterial properties, contributing to innate immunity.
• Modulates multiple aspects of the acute phase response, with levels increasing in inflammatory conditions.
• The uncleaved alpha-2 allele product (zonulin) regulates intestinal permeability by modulating tight junctions, influencing immune tolerance.
• Contains disulfide bonds, is glycosylated, and exists as a glycoprotein.
• Secreted into the plasma.
• Primarily expressed in the liver and secreted into circulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 307-406) of human haptoglobin. This region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), haptoglobin migrates at approximately 45 kDa; consider using reducing conditions to resolve the alpha and beta chains if detecting the cleaved form.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), fixation and antigen retrieval conditions should be optimized, given the secreted nature of the target.
• For ELISA, pairing this antibody with a suitable detection antibody may enable sandwich assay development for quantifying haptoglobin levels in serum or plasma.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, providing high-quality antibodies like this anti-Haptoglobin clone for hemolysis and inflammation research. We offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development. For rare or custom targets, our global supply network ensures timely access to the critical raw materials you need.