Phospho-p53-S376 Rabbit Monoclonal Antibody for WB, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Multifunctional transcription factor that acts as a tumor suppressor by inducing cell cycle arrest or apoptosis upon DNA damage. Functions by binding to specific DNA sequences to regulate target gene expression. Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Mediates apoptosis partly through transcriptional activation of pro-apoptotic genes such as BAX and FAS, and repression of anti-apoptotic Bcl-2. Pro-apoptotic activity is modulated by interactions with ASPP proteins PPP1R13B and TP53BP2, which can be displaced by the inhibitor iASPP. Related references: PMID:12524540, PMID:17189187
• Negatively regulates cell division by controlling expression of cell cycle inhibitors like cyclin-dependent kinase inhibitor p21, and can prevent CDK7 kinase activity in response to DNA damage.
• Localizes to the nucleus under normal conditions but can translocate to cytoplasm, mitochondria, and other compartments; also found in nuclear PML bodies, centrosomes, and endoplasmic reticulum, reflecting its diverse functions.
• Undergoes numerous post-translational modifications including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, which regulate its stability, DNA binding, and transcriptional activity.
• Expressed ubiquitously in tissues; multiple isoforms are generated through alternative splicing and promoter usage, some exhibiting tissue-specific expression and regulatory functions (e.g., isoform 2 enhances transactivation, isoform 4 suppresses it, isoform 7 inhibits apoptosis).
• Regulates circadian rhythm by repressing CLOCK-BMAL1-mediated activation of the PER2 gene. Related references: PMID:24051492
• Acts as a key tumor suppressor, with mutations in TP53 linked to Li-Fraumeni syndrome and many sporadic cancers, underscoring its critical role in maintaining genomic stability.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic phospho-peptide corresponding to residues around S376 of human p53. Ensure proper phosphorylation status detection by using phosphatase inhibitors during cell lysis and sample preparation.
• For Western blotting, treatment of cells with DNA-damaging agents (e.g., doxorubicin or UV irradiation) can increase p53 phosphorylation at S376, providing a positive control. The predicted molecular weight is approximately 44 kDa, but multiple bands may appear due to splice isoforms.
• In ELISA, consider using recombinant p53 protein phosphorylated at S376 as a standard and validate assay specificity with non-phosphorylated peptide controls.
• Cross-reactivity has been confirmed with human and rat p53; for other species, sequence homology analysis around S376 is advised before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized B2B partner for diagnostic and research communities, offering comprehensive IVD raw-material solutions from discovery to commercialization. For p53-related oncology and cell cycle research, we supply validated phospho-specific and pan antibodies, recombinant proteins, and ancillary reagents in bulk. Our sourcing network can also accommodate requests for rare targets, antibody pairs, and customized production, helping you accelerate assay development.