Anti-p53 (Phospho S392) Rabbit Monoclonal Antibody for WB and ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 (TP53) Function and Localization

• Multifunctional transcription factor that binds DNA to induce cell cycle arrest, DNA repair, or apoptosis (PubMed:11025664, PubMed:12524540, PubMed:12810724). Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Acts as a tumor suppressor by negatively regulating cell division through transcriptional control of target genes (PubMed:11025664, PubMed:12524540, PubMed:12810724). Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Promotes apoptosis via stimulation of BAX and FAS expression or repression of Bcl-2, modulated by interactions with ASPP family proteins (PubMed:12524540, PubMed:17189187). Related references: PMID:12524540, PMID:17189187
• Implicated in circadian clock regulation by repressing CLOCK-BMAL1-mediated PER2 transcription (PubMed:24051492). Related references: PMID:24051492
• Localizes to multiple subcellular compartments including nucleus, cytoplasm, PML bodies, endoplasmic reticulum, mitochondrion, and centrosome.
• Ubiquitously expressed, with multiple isoforms showing tissue-dependent expression patterns.
• Undergoes extensive post-translational modifications such as phosphorylation (e.g., at Ser392), acetylation, and ubiquitination, which modulate its stability and activity.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic phosphorylated peptide around Ser392 of human p53; consider using the corresponding non-phosphorylated peptide or phosphatase treatment to confirm phospho-specificity.
• For Western blot, p53 migrates at approximately 44–53 kDa due to post-translational modifications; validate the band size in your sample system.
• In ELISA applications, antibody specificity may be verified by comparing binding to phosphorylated versus non-phosphorylated p53 peptides.
• Cross-reactivity with rat p53 is reported; for other species, sequence alignment around the phosphorylation site is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material sourcing, offering end-to-end support from concept to clinic. This phospho-p53 (S392) antibody supports oncology and cell signaling research, and we provide a full suite of complementary products including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our expertise in rare target raw-material sourcing accelerates your assay development, ensuring a reliable supply chain for diagnostic manufacturers and laboratories worldwide.