Phospho-p53-S37 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence, acting as a tumor suppressor. Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Pro-apoptotic activity is mediated by stimulation of BAX and FAS antigen expression or repression of Bcl-2, and is regulated by interactions with ASPP1/ASPP2 and iASPP. Related references: PMID:12524540, PMID:17189187
• Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492
• Localizes to multiple subcellular compartments including the nucleus, cytoplasm, mitochondria, and centrosome, reflecting its diverse functional roles.
• Undergoes extensive post-translational modifications such as phosphorylation, acetylation, methylation, and ubiquitination, which modulate stability and activity.
• Ubiquitously expressed with multiple isoforms showing tissue-dependent expression; for example, isoform 2 is absent from brain and lung, while isoform 8 is restricted to colon, bone marrow, and testis.

Experimental Guidance and Technical Tips

• The antibody was generated against a phosphorylated peptide corresponding to Ser37; specificity for phosphorylated p53 (Ser37) should be confirmed using phosphatase-treated samples or non-phosphorylated peptide blocking.
• For Western blot (WB), consider using denaturing and reducing conditions; include phosphatase inhibitors during sample preparation to preserve phosphorylation signals.
• When performing immunohistochemistry (IHC-P), antigen retrieval methods and the use of phosphatase inhibitors may help retain the phospho-signal; validate detection in the relevant tissue type.
• Cross-reactivity with human, mouse, and rat suggests sequence conservation around Ser37; verify performance in your experimental system.
• ELISA can be used to quantify phospho-p53-S37 levels; appropriate positive and negative controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies like this phospho-p53-S37 antibody, and ancillary reagents for diagnostic development. From concept to clinic, we support researchers and manufacturers in securing rare-target materials and large-scale bulk supplies. Whether you need phospho-specific detection reagents or full workflows, CamelBio simplifies procurement for your p53-related research and assay needs.