Phospho-p53-S6 Rabbit pAb - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Cellular tumor antigen p53 is a multifunctional transcription factor and tumor suppressor that induces cell cycle arrest, DNA repair, or apoptosis in response to cellular stress.
  Related references: PMID:11025664, PMID:12524540, PMID:12810724
• p53 negatively regulates cell division by activating genes such as cyclin-dependent kinase inhibitors and controls apoptosis via modulation of BAX, FAS, and Bcl-2 expression.
  Related references: PMID:12524540, PMID:17189187
• The protein is encoded by the TP53 gene, also known by aliases BCC7, LFS1, BMFS5, and TRP53, with multiple isoforms generated through alternative splicing and promoter usage.
• Subcellularly, p53 shuttles between the cytoplasm and nucleus, and localizes to the PML body, endoplasmic reticulum, mitochondrion matrix, centrosome, and other compartments, reflecting its diverse functions.
• p53 is subject to extensive post-translational modifications including phosphorylation, acetylation, ubiquitination, and methylation, which regulate its stability and activity; the phosphorylation at serine 6 (S6) is an important regulatory event.
• As a tumor suppressor, p53 is implicated in Li-Fraumeni syndrome and various cancers, and interacts with viral proteins, highlighting its role in host-virus interactions.
• p53 also plays a role in regulating circadian rhythm by repressing CLOCK-BMAL1-mediated PER2 transcription.
  Related references: PMID:24051492
• Tissue expression is ubiquitous, with specific isoforms showing tissue-dependent patterns; for example, isoform 2 is absent in brain and lung, while isoform 8 is restricted to colon, bone marrow, and a few other tissues.

Experimental Guidance and Technical Tips

• For Western blot, validate specificity using phosphatase-treated lysates or peptide competition with phosphorylated versus non-phosphorylated peptides to confirm phospho-S6 recognition.
• In ELISA applications, consider coating the plate with a phospho-peptide corresponding to the S6 region to enhance detection sensitivity and specificity.
• Given ubiquitous p53 expression, select appropriate cell lines or tissue samples and include phosphatase inhibitors during lysis to preserve phosphorylation signals; optimize loading amounts and antibody dilutions for each experimental system.
• As a rabbit polyclonal antibody, expect robust signal but verify lot-to-lot consistency and cross-reactivity in your sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For p53-focused assay development, we provide phospho-specific detection tools like this Phospho-p53-S6 antibody, supporting research into apoptosis, cell cycle, and cancer biology. Our supply chain network also assists with rare target sourcing to accelerate your diagnostic pipeline.