Anti-Phospho-p53 (Ser15) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 functions as a tumor suppressor transcription factor, inducing cell cycle arrest, DNA repair, or apoptosis in response to cellular stress. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• Apoptosis is mediated via upregulation of BAX and FAS, and downregulation of Bcl-2. Related references: PMID:12524540 PMID:17189187
• Pro-apoptotic activity is enhanced by ASPP1/ASPP2 and suppressed by iASPP. Related references: PMID:12524540
• Involved in circadian rhythm regulation by repressing PER2 transcription. Related references: PMID:24051492
• Subcellular localization includes nucleus, cytoplasm, mitochondria, endoplasmic reticulum, and centrosome.
• Ubiquitously expressed, with tissue-dependent isoform patterns.
• Extensively modified by phosphorylation, acetylation, methylation, and ubiquitination, which control its stability and activity.

Experimental Guidance and Technical Tips

• For Western blotting (WB), expect a band at approximately 44 kDa corresponding to phospho-p53 (Ser15).
• Confirm antibody specificity by using non-phosphorylated peptide or phosphatase treatment as negative controls.
• Suitable for immunofluorescence (IF/ICC) and ELISA; optimize dilutions for your experimental setup.
• The antibody cross-reacts with mouse p53, enabling studies in mouse models.
• Since p53 phosphorylation is often induced by DNA damage, consider appropriate treatment conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-phospho-p53 (Ser15) antibody is ideal for cancer research and apoptosis studies. Leverage CamelBio's extensive supply portfolio, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, for your IVD development needs. Our sourcing expertise ensures efficient delivery of even rare-target raw materials.