Phospho-p53-S33 Rabbit mAb for WB, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Encoded by the TP53 gene, cellular tumor antigen p53 is a phosphoprotein that exists as multiple isoforms due to alternative splicing and promoter usage.
• Functions as a multifunctional transcription factor that induces cell cycle arrest, DNA repair, or apoptosis upon DNA damage. Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Acts as a crucial tumor suppressor by negatively regulating cell division through control of gene expression. Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Promotes apoptosis via stimulation of BAX and FAS antigen expression or repression of Bcl-2, with activity modulated by interaction with ASPP proteins. Related references: PMID:12524540, PMID:17189187
• Localizes to multiple subcellular compartments, including nucleus, cytoplasm, mitochondria, centrosome, and endoplasmic reticulum, consistent with its diverse regulatory roles.
• Subject to extensive post-translational modifications, including phosphorylation at serines such as S33, acetylation, methylation, and ubiquitination, which govern stability and transcriptional activity.
• Ubiquitously expressed with tissue-dependent isoform distribution; certain isoforms may exhibit dominant-negative effects on canonical p53 functions.
• Regulates circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492

Experimental Guidance and Technical Tips

• Because this antibody targets phosphorylation at S33, add phosphatase inhibitors to lysis buffers and handle samples on ice to preserve phosphorylation status.
• For Western blot, consider using a phospho-specific blocking regimen and confirm specificity by comparing signals before and after phosphatase treatment or peptide competition.
• In ELISA, capture or detect phospho-p53 in cell lysates; include non-phosphorylated and untreated controls to validate phospho-specificity.
• p53 can be low in abundance under normal conditions; enrichment by immunoprecipitation or treatment with DNA-damaging agents may improve detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. This anti-phospho-p53-S33 rabbit monoclonal antibody strengthens our portfolio for apoptosis and cell cycle biomarker detection, supporting immunoassay development for cancer research. We also supply validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your project from concept to clinic.