Anti-Phospho-p53 (T55) Rabbit Monoclonal Antibody for WB and ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53, also known as Cellular tumor antigen p53, Phosphoprotein p53, and Tumor suppressor p53, is a multifunctional transcription factor encoded by the TP53 gene. It plays a critical role in tumor suppression by inducing cell cycle arrest, DNA repair, or apoptosis in response to cellular stress. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• One of its key mechanisms is the negative regulation of cell division by controlling the expression of genes required for this process, including activation of a cyclin-dependent kinase inhibitor. This leads to cell cycle arrest at the G1/S checkpoint. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• p53 induces apoptosis through stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is enhanced by interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 and inhibited by PPP1R13L/iASPP. Related references: PMID:12524540 PMID:17189187
• The protein localizes to multiple subcellular compartments including the nucleus, cytoplasm, mitochondria, endoplasmic reticulum, and centrosomes. This dynamic distribution is linked to its diverse regulatory functions in transcription, apoptosis, and metabolism.
• p53 undergoes extensive post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, which modulate its stability, DNA-binding affinity, and transcriptional activity. The threonine 55 (T55) phosphorylation site is relevant for studying p53 activation signaling.
• Isoforms of p53 (isoforms 2, 3, 4, 7, 8, 9) exhibit tissue-dependent expression and distinct regulatory functions, such as enhancing or suppressing the transactivation activity of isoform 1, contributing to the complexity of p53 signaling networks.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phosphopeptide corresponding to T55 of human p53. It is designed to detect phosphorylation at this site; consider using phosphatase-treated samples as a negative control to confirm specificity.
• For western blotting (WB), validate against lysates from cells known to express phospho-p53 under DNA damage conditions (e.g., treatment with etoposide or UV irradiation). The predicted molecular weight is ~44 kDa, but phosphorylated forms may exhibit slight shifts.
• In ELISA applications, immobilize the antibody or the peptide antigen appropriately and include non-phosphorylated peptide controls to assess phospho-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents tailored for diagnostic development. Targeting tumor suppressor p53, this phospho-T55 specific antibody supports oncology research and assay design. From rare target sourcing to technical consulting, CamelBio bridges the gap between concept and clinic.