Phospho-NFKB1-S337 Rabbit PolymAb® for WB - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor, which undergoes proteasome-mediated processing to generate the active p50 subunit of the NF-κB transcription factor complex (PubMed:1423592). Related references:
  PMID:1423592

• The p50 subunit forms homo- or heterodimers with other NF-κB/Rel family members such as RELA/p65, RELB, and REL, binding the κB consensus DNA sequence 5'-GGRNNYYCC-3' to regulate gene expression (PubMed:1740106, PubMed:7830764). Related references:
  PMID:1740106
  PMID:7830764

• NF-κB is a central mediator of immune and inflammatory responses, cell growth, differentiation, and apoptosis, with the p65-p50 heterodimer being the most abundant and transcriptionally activating complex.

• The inactive NF-κB dimers are retained in the cytoplasm by IκB inhibitors; upon stimulation, IκB is phosphorylated and degraded, allowing NF-κB to translocate to the nucleus and activate target genes.

• NFKB1 also functions as a cytoplasmic retention anchor via p105 for associated NF-κB proteins and, in complex with MAP3K8, represses MAPK signaling until p105 degradation releases active MAP3K8.

• The protein is subject to multiple post-translational modifications including phosphorylation, acetylation, ubiquitination, and hydroxylation, which modulate its activity, stability, and subcellular localization.

• Subcellular localization includes both cytoplasm and nucleus, reflecting its dual role in signal sequestration and transcriptional regulation.

• Alternative isoforms produced by alternative splicing contribute to functional diversity; the full-length p105 protein (105 kDa) and the processed p50 subunit (50 kDa) are detected in most cell types.

Experimental Guidance and Technical Tips

• Given the phospho-specific nature of this antibody, it is essential to include phosphatase inhibitors in lysis buffers to preserve the phosphorylation signal during sample preparation.
• For Western blot, a positive control with known NF-κB pathway activation (e.g., TNF-α-treated cells) is recommended; detection of bands around 105 kDa (p105) and 50 kDa (p50) should be verified.
• In immunohistochemistry, antigen retrieval conditions and antibody dilution should be optimized; ensure that the staining pattern reflects expected nuclear/cytoplasmic localization upon pathway activation.
• The antibody is suitable for ELISA; consider using phosphorylated peptide as a standard for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a single-source solution for IVD raw materials, including this Phospho-NFKB1-S337 antibody and a wide portfolio of NF-κB pathway-related reagents. Supporting projects from concept to clinic, we provide validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents, ensuring consistent supply for high-performance immunoassays. Our expertise in rare target sourcing and custom development enables streamlined procurement for your NF-κB research and diagnostic applications.