[KO Validated] Anti-p53 Rabbit Monoclonal Antibody for WB, IP, IF, IHC, ChIP, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 is a multifunctional transcription factor that induces cell cycle arrest, DNA repair, or apoptosis upon binding to target DNA sequences. Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Acts as a tumor suppressor in many tumor types, negatively regulating cell division by controlling expression of cell-cycle genes. Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Apoptosis induction is mediated by stimulation of BAX and FAS antigen expression or repression of Bcl-2; pro-apoptotic activity is regulated via interactions with ASPP proteins. Related references: PMID:12524540, PMID:17189187
• Multiple isoforms exist with distinct functions: isoform 2 enhances transactivation activity of isoform 1, isoform 4 suppresses growth suppression, and isoform 7 inhibits apoptosis.
• Subcellular localization includes cytoplasm, nucleus, PML body, endoplasmic reticulum, mitochondrial matrix, and centrosome, reflecting its diverse roles.
• Ubiquitously expressed, with isoform-specific tissue distribution; for example, isoform 2 is absent in brain and lung while isoform 8 is detected only in colon, bone marrow, testis, fetal brain, and intestine.
• Implicated in circadian clock regulation by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492
• Post-translational modifications include acetylation, phosphorylation, methylation, ubiquitination, and sumoylation, modulating stability, activity, and localization (Keywords: Acetylation, Activator, Alternative splicing, Apoptosis, Biological rhythms, Cell cycle, Disease variant, DNA-binding, Glycoprotein, Isopeptide bond, Metal-binding, Methylation, Necrosis, Phosphoprotein, Reference proteome, Repressor, Transcription, Transcription regulation, Tumor suppressor, Ubl conjugation, Zinc).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-393 of human p53 (full-length wild-type). Consider verifying linear epitope recognition if native conformation is critical.
• The observed molecular weight range of 24-44 kDa accounts for multiple isoforms and post-translational modifications; validate the expected band size in your specific sample type.
• Suitable for WB, IP, IF/ICC, IHC-P, ChIP, and ELISA applications; optimize antibody dilutions and choose appropriate positive controls (e.g., p53-expressing cell lines) for each technique.
• Cross-reactivity is confirmed for human; when working with other species, check sequence homology and perform a pilot experiment.

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