Anti-Phospho-p53-S392 Rabbit Polyclonal Antibody for WB, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 is a multifunctional transcription factor that induces cell cycle arrest, DNA repair, or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724). Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed:11025664, PubMed:12524540, PubMed:12810724). Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Apoptosis induction is mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Related references: PMID:12524540, PMID:17189187
• Its pro-apoptotic activity is activated via interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2, and inhibited by PPP1R13L/iASPP (PubMed:12524540). Related references: PMID:12524540
• Localizes to multiple subcellular compartments including nucleus, cytoplasm, mitochondria, and endoplasmic reticulum, essential for its diverse functions.
• Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). Related references: PMID:24051492
• Ubiquitously expressed with tissue-dependent isoform expression, and subject to extensive post-translational modifications such as phosphorylation, acetylation, and ubiquitination; phosphorylation at S392 modulates its activity.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to the phosphorylated S392 residue of human p53, ensuring specificity for the phosphorylated form.
• For Western blot (WB) applications, consider including phosphatase inhibitor cocktails in lysis buffers and validating specificity using phosphatase treatment or non-phosphorylated peptide blocking.
• In ELISA, the antibody can serve as a capture or detection reagent; optimize coating conditions and confirm linearity with phosphorylated p53 standards.
• The antibody cross-reacts with human, mouse, and rat p53, enabling cross-species studies in relevant model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This Phospho-p53-S392 rabbit polyclonal antibody supports p53 signaling and apoptosis research applications. With a network spanning rare target sourcing, CamelBio bridges the gap from concept to clinic, ensuring reliable procurement for your assay development needs.