Phospho-PPIG-S376 Rabbit pAb for WB, ELISA - Q13427

Core Product Specifications and Parameters

Biological Background: PPIG Function and Localization

• Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as cyclophilin G (CypG) or CARS-cyclophilin, is a member of the cyclophilin family of peptidyl-prolyl isomerases that catalyze proline cis-trans isomerization.
• PPIG specifically catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, which may assist protein folding.
  Related references: PMID:20676357
• Plays a regulatory role in pre-mRNA splicing, likely through its interaction with the spliceosome and localization to nuclear speckles.
• Primarily localized to the nuclear matrix and nuclear speckles, subnuclear compartments involved in RNA processing.
• Expressed ubiquitously across human tissues.
• Subject to phosphorylation at serine 376 and other residues, which may modulate its isomerase activity or subcellular distribution.
• As a phosphoprotein, PPIG is also implicated in proteomics identification studies and may undergo alternative splicing and ubiquitin-like modification.

Experimental Guidance and Technical Tips

• To confirm specificity for the phosphorylated epitope, consider comparing signals from phosphatase-treated versus untreated lysates in your western blot experiments.
• The predicted molecular weight of PPIG is 89 kDa; a band near this size may be observed in appropriate sample systems.
• This antibody cross-reacts with human, mouse, and rat PPIG according to the supplier; validate in the relevant species to ensure performance.
• For ELISA, the phosphorylated peptide immunogen should be used as the coating antigen to maintain epitope recognition.

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