Anti-Phospho-p53 (S33) Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Encoded by the TP53 gene, cellular tumor antigen p53 is a multifunctional transcription factor that acts as a potent tumor suppressor by regulating cell cycle arrest, DNA repair, and apoptosis in response to various cellular stresses. Related references: PMID:11025664 PMID:12524540 PMID:12810724

• p53 exerts its pro-apoptotic functions through both transcriptional activation (e.g., BAX and FAS) and repression (e.g., Bcl-2), with its activity modulated by interactions with ASPP family proteins. Related references: PMID:12524540 PMID:17189187

• Beyond nuclear transcription, p53 also localizes to the cytoplasm, mitochondria, endoplasmic reticulum, and centrosomes, reflecting its involvement in non-transcriptional apoptosis and necrosis.

• p53 is ubiquitously expressed across normal human tissues, with several alternative isoforms exhibiting tissue-dependent expression patterns.

• Key post-translational modifications include phosphorylation, acetylation, methylation, and ubiquitination, which tightly regulate p53 stability, subcellular localization, and transcriptional activity.

• p53 contributes to circadian clock regulation by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Ser33 of human p53. To validate phospho-specificity, consider using lambda phosphatase-treated samples or non-phosphopeptide competition in parallel.
• For western blot (WB), predicted band size is ~53 kDa (canonical p53) or 44 kDa (some isoforms); bands may shift slightly due to phosphorylation and other modifications.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and tissue fixation can affect phospho-epitope detection; optimize using known positive controls such as irradiated or genotoxic-stress treated tissues.
• When performing immunoprecipitation (IP) or ELISA, include appropriate IgG controls and consider pairing with a total p53 antibody to assess phosphorylation stoichiometry.
• This antibody cross-reacts with mouse and rat p53, making it suitable for translational studies; however, always confirm cross-reactivity in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with validated IVD raw materials essential for p53-related studies, including phospho-specific antibodies, antibody pairs, and ancillary reagents. With a focus on streamlining procurement from early-stage biomarker discovery to clinical assay development, CamelBio offers bulk supply options, rigorous lot-to-lot consistency, and custom sourcing for rare targets. Let CamelBio be your reliable partner in accelerating immunoassay and companion diagnostic development.