Phospho-p53-S367 Rabbit Polyclonal Antibody for WB and ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 is a multifunctional transcription factor that induces cell cycle arrest, DNA repair, or apoptosis upon DNA binding, acting as a critical tumor suppressor. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• Apoptosis induction is mediated through stimulation of BAX and FAS antigen expression or repression of Bcl-2, with regulation by ASPP family proteins. Related references: PMID:12524540 PMID:17189187
• Involved in oxidative stress-induced necrosis via cooperation with mitochondrial PPIF, largely independent of transcription.
• Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492
• Localizes to multiple subcellular compartments including nucleus, cytoplasm, mitochondria, endoplasmic reticulum, and centrosome, reflecting its diverse functions.
• Subject to various post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, which modulate its stability and activity.
• Expressed ubiquitously, but multiple isoforms exhibit tissue-dependent expression patterns, with some isoforms having distinct transcriptional regulatory activities.
• Keywords highlight its roles in apoptosis, cell cycle, transcription regulation, tumor suppression, and as a phosphoprotein.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic phosphorylated peptide around Ser367, the antibody is expected to specifically recognize p53 only when phosphorylated at this residue. Consider using phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, validate the antibody with both phosphorylated and non-phosphorylated p53 controls (e.g., using phosphatase treatment) to confirm specificity.
• The antibody is recommended for ELISA; appropriate peptide blocking experiments may help confirm phospho-specific binding.
• It is advisable to optimize antibody concentration for each experimental system, and to include relevant positive controls such as DNA damage-treated cells known to induce p53 phosphorylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, technical services, and consulting—from concept to clinic. This phospho-p53-S367 antibody supports research into cancer biology and diagnostic assay development. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets to streamline your procurement.