Anti-p53 Rabbit Monoclonal Antibody (KO Validated) for WB, IHC-P, IF/ICC - P02340

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Cellular tumor antigen p53 is a multifunctional transcription factor that regulates cell cycle arrest, DNA repair, and apoptosis upon DNA damage, acting as a critical tumor suppressor. Related references: PMID:19556538, PMID:20673990, PMID:22726440
• p53 mediates apoptosis through transcriptional activation of BAX and FAS, or repression of Bcl-2, and its pro-apoptotic activity is modulated by ASPP family proteins.
• In response to DNA damage, p53 inhibits CDK7 kinase activity within the CAK complex, halting cell cycle progression.
• p53 localizes to multiple subcellular compartments including the nucleus, cytoplasm, PML bodies, endoplasmic reticulum, and mitochondria, reflecting its diverse functional roles.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate p53 stability and activity.
• p53 regulates circadian rhythms by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492
• p53 induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21), which participates in p53-dependent transcriptional repression and apoptosis.
• In cooperation with mitochondrial PPIF, p53 activates oxidative stress-induced necrosis in a transcription-independent manner.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide, suggesting the antibody likely targets a linear epitope conserved across human, mouse, and rat. When using for Western blot, consider optimizing detection conditions under reducing and non-reducing conditions.
• For IHC-P, antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate buffer) may enhance staining; validate in appropriate positive and negative tissue controls.
• In IF/ICC, fixation and permeabilization protocols should be tested, as p53 can be present in both nuclear and cytoplasmic compartments depending on cell type and stress conditions.
• For IP and ELISA applications, confirm antibody performance with known p53-expressing lysates and recombinant p53 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with a streamlined supply chain for critical raw materials like the p53 antibody, supporting cancer and apoptosis research applications. We offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your diagnostic development from concept to clinic.