Phospho-p53-T18 Rabbit Polyclonal Antibody for WB, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53, encoded by the TP53 gene, is a cellular tumor antigen and a critical tumor suppressor that functions as a multifunctional transcription factor.
  Related references:
  PMID:11025664, PMID:12524540, PMID:12810724

• Upon binding to its target DNA sequence, p53 induces cell cycle arrest, DNA repair, or apoptosis, thereby negatively regulating cell division.
  Related references:
  PMID:11025664, PMID:12524540, PMID:12810724

• Apoptosis induction is mediated via stimulation of BAX and FAS expression or repression of Bcl-2, and is modulated by interactions with ASPP1/ASPP2 (activation) and iASPP (inhibition).
  Related references:
  PMID:12524540, PMID:17189187

• p53 shuttles between the cytoplasm and nucleus, and localizes to PML bodies, endoplasmic reticulum, mitochondria, and centrosomes, reflecting its diverse functional roles.

• Ubiquitously expressed, with multiple isoforms showing tissue-dependent distribution patterns.

• As a phosphoprotein, p53 undergoes extensive post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its stability and activity; phosphorylation at Thr18 is a key event in its activation.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using lysis buffers containing phosphatase inhibitors to preserve the phosphorylation signal at Thr18.
• Validate antibody specificity by comparing signals from phosphatase-treated samples or by competitive blocking with the non-phosphorylated peptide.
• Since the antibody is raised in rabbit, pair with an appropriate anti-rabbit secondary antibody for detection.
• When performing ELISA, ensure plate coating with the phosphorylated peptide or antigen and optimize antibody dilution.
• Confirm cross-reactivity in additional species if required, as the current validation is for human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for diagnostic manufacturers and laboratories, offering comprehensive access to IVD raw materials. This anti-phospho-p53 (Thr18) rabbit polyclonal antibody supports cancer research and diagnostic assay development. Rely on CamelBio for validated antibody pairs, optimized antibodies, and ancillary reagents to streamline your workflow from concept to clinic.