Asymmetric DiMethyl-Histone H4-R3 Rabbit pAb | P62805 | Validated for WB, IF/ICC, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, around which DNA is wrapped to form chromatin. This fundamental structure compacts genomic DNA and regulates its accessibility to enzymes and other factors required for DNA-templated processes such as transcription, replication, and repair. Histones, including H4, are therefore central to maintaining chromosomal stability and epigenetic control.

A complex set of post-translational modifications, often referred to as the histone code, regulates chromatin dynamics. Histone H4 undergoes numerous modifications, including acetylation, methylation, phosphorylation, and citrullination, at various residues along its N-terminal tail and globular domain.

This antibody specifically targets the asymmetric dimethylation of arginine 3 (R3) in the N-terminal tail of histone H4. In the processed protein, this corresponds to residue 4 (R4). This modification is catalyzed primarily by protein arginine methyltransferase 1 (PRMT1) and plays a role in transcriptional activation and chromatin remodeling.

Related references: PMID:11387442, PMID:11448779, PMID:15345777

Histone H4 is localized to the nucleus and chromosomes. During spermatogenesis, acetylation of H4 is associated with its nuclear localization in step 11 spermatids.

Related references: UniProtKB P62805

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide carrying asymmetric dimethylation at R3, ensuring high specificity for the methylated form of histone H4. Consider validating specificity by comparing signals from methylated versus non-methylated peptide competition assays.
• For Western blot (WB), histone extraction protocols that enrich for nuclear or chromatin-bound proteins are recommended. The predicted molecular weight of histone H4 is approximately 11 kDa, though it may migrate slightly higher or as a band depending on the extent of other modifications and gel conditions.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), expect nuclear staining. Permeabilization and fixation conditions may need optimization to preserve epitope recognition while exposing the nuclear compartment.
• When using this antibody in ELISA, consider coating with methylated peptide or histone extracts to screen for binding activity. Always include appropriate controls to rule out non-specific binding.
• This antibody has been reported to detect H4 from human, mouse, and rat, with a wide range of other species predicted. Cross-reactivity should be verified in the relevant sample system if working with non-mammalian models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-asymmetric dimethyl-histone H4-R3 (P62805) Rabbit pAb is part of our extensive portfolio for epigenetic and chromatin research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to secure your supply chain for high-quality IVD components.