Asymmetric DiMethyl-Histone H3-R2 Rabbit Polyclonal Antibody (Q16695/P68431) for WB and ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, essential for compacting DNA into chromatin and regulating gene expression, DNA repair, replication, and chromosomal stability. Related references: UniProt Q16695, UniProt P68431.
• Post-translational modifications of histone H3, particularly on its N-terminal tail, form a “histone code” that governs chromatin dynamics. Asymmetric dimethylation at arginine 2 (H3R2me2a) is catalyzed by protein arginine methyltransferase 6 (PRMT6). This modification is implicated in transcriptional regulation and chromatin structure. Related references: PMID:17898714, PMID:18077460, PMID:18079182.
• Histone H3 localizes to the nucleus and associates with chromosomes throughout the cell cycle.
• Two major variants are recognized: the replication-dependent H3.1 (encoded by genes such as HIST1H3A, P68431) and the testis-specific H3.1t (H3-4, Q16695), which is expressed in testicular cells.
• The immunogen corresponds to a peptide with asymmetric dimethylation at the first arginine after initiator methionine removal (position 3 in the full-length protein), ensuring specific recognition of the H3R2me2a mark.

Experimental Guidance and Technical Tips

• Confirm antibody specificity by using non-methylated or symmetrically dimethylated peptide competitors in your assay.
• For western blot (WB), whole-cell lysates typically show a band at approximately 15 kDa. Optimize primary antibody dilution and consider loading controls such as total H3 or pan-histone H3 antibodies.
• In ELISA, use the immunizing peptide as a positive control and test against peptides with different modifications to verify selectivity.
• Due to high histone H3 sequence conservation, cross-reactivity is predicted with many species; however, validate reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop sourcing platform for IVD raw materials, from concept to clinic. For researchers studying epigenetic modifications and chromatin biology, we provide high-quality antibodies like this Asymmetric DiMethyl-Histone H3-R2 pAb, along with validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and customized rare-target sourcing. Partner with us to streamline your procurement and accelerate your diagnostic development workflow.