Symmetric DiMethyl-Histone H3-R8 (Q16695/P68431) Rabbit mAb for Western Blot, IHC, and IF/ICC

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications, often termed the "histone code."
  Related references: UniProt Q16695

• Histone H3 localizes predominantly to the nucleus and is integral to chromosome structure.
  Related references: UniProt Q16695

• Histone H3 undergoes extensive post-translational modifications at its N-terminal tail, including acetylation, methylation, phosphorylation, and citrullination, among others. The symmetric dimethylation of arginine 8 (R8, corresponding to R9 in the canonical UniProt sequence) is catalyzed by protein arginine methyltransferase 5 (PRMT5) and is a conserved epigenetic mark associated with transcriptional regulation.
  Related references: UniProt P68431

• The H3.1t isoform (Q16695) is testis-specific, while the canonical H3.1 (P68431) is ubiquitously expressed.
  Related references: UniProt Q16695

Experimental Guidance and Technical Tips

• The immunogen is a synthetic symmetric dimethylated peptide around R8 of human Histone H3; therefore, confirm the antibody's specificity for the symmetric dimethyl mark in your assay system, as it may not recognize asymmetric dimethylation or unmethylated histone.
• For Western blotting, a band around 16 kDa is expected; use appropriate histone extraction protocols to enrich chromatin proteins.
• This antibody has been validated for multiple applications including WB, DB, IHC-P, IF/ICC, and ELISA. Consider optimizing dilutions and conditions based on your experimental setup.
• The reported cross-reactivity covers human, mouse, rat, and wide-range predicted species. If working with non-standard species, validate reactivity empirically.
• For IHC, note that antigen retrieval may be required; test fixation and permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop provider of IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutes access to validated antibodies, antigen pairs, and ancillary reagents. This symmetric dimethyl-histone H3-R8 antibody supports epigenetics and chromatin biology research, and CamelBio can further assist with bulk orders, customized antibody panels, and sourcing of rare-target reagents to accelerate your diagnostic development. Partner with us for reliable supply from concept to clinic.