DiMethyl-Histone H3-K27 Rabbit Polyclonal Antibody (Q16695/P68431) for Epigenetic Research & ChIP

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin. Nucleosomes wrap and compact DNA, limiting accessibility to the cellular machinery and thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.

Histone H3 is subject to a vast array of post-translational modifications (PTMs), including acetylation, methylation, phosphorylation, citrullination, and many others, collectively referred to as the "histone code." These modifications regulate chromatin structure and gene expression dynamically. The N-terminal tail of histone H3 is particularly rich in modifiable residues, with lysine 27 (K27) being a critical site for methylation events. Di-methylation of H3K27 (H3K27me2) is associated with transcriptional repression and heterochromatin maintenance.

The human genome encodes several histone H3 variants. Q16695 (H3-4, also known as H3.1t) is a testis-specific histone primarily expressed in testicular cells, while P68431 (H3C1/H3C2/H3C3/H3C4/H3C6/H3C7/H3C8/H3C10/H3C11/H3C12) represents the canonical replication-dependent H3.1 variants expressed in somatic cells. Both variants are highly conserved and share identical N-terminal sequences where K27 is located. They localize exclusively to the nucleus and chromosomes, where they form an integral part of chromatin fibers.

Related references: UniProt Q16695, UniProt P68431

Experimental Guidance and Technical Tips

• For Western blot (WB), a single band around 15 kDa corresponding to histone H3 is expected. Use appropriate positive and negative controls to confirm band identity and dimethylation specificity.
• For ChIP and ChIP-seq, optimize cross-linking (e.g., formaldehyde) and chromatin shearing conditions. Consider validating enrichment of known H3K27me2 target loci using qPCR or parallel sequencing.
• The antibody cross-reacts with human, mouse, and rat, and is predicted to have a wide range of reactivity. However, it is advisable to validate performance in the specific species and tissue of interest before large-scale experiments.
• For ELISA and dot blot (DB), titrate the antibody and include blocking steps to minimize non-specific binding; the peptide immunogen can serve as a positive control.
• In immunohistochemistry (IHC) and immunofluorescence (IF/ICC), note that histone H3 is nuclear; a counterstain (e.g., DAPI) can help visualize nuclear localization. Antigen retrieval may be necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD manufacturers and research laboratories with a comprehensive, one-stop supply chain for raw materials, from concept to clinic. Along with this DiMethyl-Histone H3-K27 antibody for epigenetics and chromatin research, we offer a complete portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities. Partner with CamelBio to streamline your diagnostic raw material procurement and accelerate your assay development.