DiMethyl-Histone H4-K20 Rabbit pAb (P62805) for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, wrapping and compacting DNA into chromatin, which limits DNA accessibility for transcription, DNA repair, DNA replication, and chromosomal stability.
• It plays a central role in the histone code, where extensive post-translational modifications (PTMs) at specific residues regulate chromatin dynamics. Modifications include acetylation, methylation, phosphorylation, citrullination, and ubiquitination.
• Lysine 20 dimethylation (H4K20me2) is a well-conserved epigenetic mark associated with constitutive heterochromatin formation, transcriptional silencing, DNA replication, and the DNA damage response.
• Histone H4 localizes to the nucleus and chromosomes. Related references: PMID:12086618, PMID:15964846

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic dimethylated peptide spanning lysine 20 of human histone H4, providing specific recognition of the H4K20me2 modification.
• For Western blotting (WB), the expected molecular weight is approximately 11 kDa; include appropriate molecular weight markers and consider using nuclear extracts for enriched target detection.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), permeabilization steps and antigen retrieval conditions may influence staining; validate the antibody in your specific tissue or cell system alongside methylation-state controls.
• For ELISA, consider using a peptide competition assay to confirm modification specificity and optimize coating concentrations.
• As this is a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative applications.
• Cross-reactivity is broad, but species-specific sequence variations at the epitope may affect signal; validate in the relevant model organism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing optimized polyclonal and monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents for diagnostic development. This H4K20me2-specific antibody supports epigenetic and chromatin research applications, and we can assist with large-scale supply, custom antibody development, and technical consulting to accelerate your assay from concept to clinic.