DiMethyl-Histone H4-K20 Rabbit Monoclonal Antibody (P62805) – Validated for WB, DB, IHC-P, ELISA in Epigenetics Studies

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is one of the four core histones (H2A, H2B, H3, H4) that form the nucleosome, the fundamental unit of chromatin. It plays a central role in compacting DNA and regulating DNA accessibility for transcription, replication, repair, and chromosomal stability. Related references: UniProt P62805

• Histone H4 is subject to extensive post-translational modifications (PTMs) including acetylation, methylation, phosphorylation, citrullination, and others. These PTMs constitute the "histone code", which dynamically regulates chromatin structure and gene expression. Related references: UniProt P62805

• The methylations at lysine 20 (K20, often referred to as K21 in some numbering systems) of Histone H4 are critical epigenetic marks. Dimethylation of K20 (H4K20me2) is associated with DNA damage response, gene silencing, and chromatin compaction. The modification is catalyzed by histone methyltransferases such as SET8/PR-Set7 and is recognized by reader proteins involved in chromatin maintenance. Related references: PMID:12086618, PMID:15964846, PMID:17967882

• Histone H4 is primarily localized in the nucleus and associated with chromosomes. Its subcellular distribution can be influenced by acetylation status, as seen in spermatids where it localizes to the nucleus only when acetylated. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• Consider using a synthetic peptide corresponding to the dimethylated K20 sequence as a blocking control to confirm specificity in Western blot or ELISA assays.
• The antibody is recommended for WB, DB, IHC-P, and ELISA applications. Determine optimal working dilutions empirically in the relevant sample system.
• Cross-reactivity is predicted for a wide range of species due to high sequence conservation of Histone H4; validate in species of interest prior to routine use.
• For IHC-P, antigen retrieval methods may need optimization; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting. This DiMethyl-Histone H4-K20 rabbit monoclonal antibody exemplifies our commitment to providing high-quality methylation-specific antibodies for epigenetic IVD assay development. Explore our portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities to accelerate your diagnostic projects from concept to clinic.