Anti-Histone H4 Rabbit PolymAb® for WB, IF/ICC, IHC-P, ChIP, ELISA - P62805

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is one of the four core histone proteins (H2A, H2B, H3, H4) that form the nucleosome octamer, wrapping ~147 bp of DNA and compacting the genome into chromatin.
• As a nucleosome core component, H4 plays a central role in regulating DNA accessibility for transcription, replication, repair, and maintaining chromosomal stability.
• In humans, H4 is encoded by 14 nearly identical genes (H4C1–H4C16) clustered at multiple chromosomal loci, producing a conserved 103-amino acid protein.
• H4 localizes exclusively to the nucleus and chromosomes, where it is subject to extensive post-translational modifications (acetylation, methylation, phosphorylation, citrullination, ubiquitination, etc.) that constitute the "histone code" to govern chromatin dynamics.
• Modifications on the N-terminal tail (aa 1–20) are critical for gene regulation; for example, acetylation at specific lysines correlates with active transcription, while methylation can signal either activation or repression depending on the residue.
• Disruptions in H4 genes or regulatory marks are linked to disease; rare mutations cause neurodevelopmental phenotypes including intellectual disability, underscoring the essential nature of histone H4 in development.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1–100, encompassing the N-terminal tail and globular domain; consider that N-terminal epitope recognition may be affected by dense post-translational modifications in some chromatin contexts.
• For Western blot (WB), a strong band is expected at ~11 kDa; include acid-extracted histone samples as positive controls to enrich for basic proteins.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), chromatin compacted in heterochromatic regions may mask the epitope; gentle DNAse digestion or mild permeabilization protocols can improve detection.
• For chromatin immunoprecipitation (ChIP), validate the antibody’s ability to enrich modified vs. unmodified nucleosomes under cross-linking and native conditions, as epitope accessibility varies with chromatin compaction.
• Cross-reactivity with mouse and rat is expected given the high sequence conservation (human/mouse H4 are identical); however, always confirm specificity in the relevant species and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for diagnostic manufacturers, laboratories, and researchers by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target procurement. This anti-Histone H4 antibody supports epigenetic and chromatin-focused assays, from basic research through translational development. Partner with CamelBio for end-to-end technical services and consulting to accelerate your project from concept to clinic.