DiMethyl-Histone H3-K4 (Q16695/P68431) Rabbit Monoclonal Antibody for ChIP, WB, and Epigenetic Research

Core Product Specifications and Parameters

Biological Background: Histone H3 (Dimethyl-K4) Function and Localization

• Histone H3 is a core component of the nucleosome, the basic unit of chromatin. Together with histones H2A, H2B, and H4, it packages DNA, regulating access for transcription, replication, repair, and chromosomal stability.
  Related references: UniProt Q16695, UniProt P68431

• Histone H3 localizes to the nucleus and chromosomes.
  Related references: UniProt Q16695, UniProt P68431

• Multiple histone H3 variants exist. The replication-dependent H3.1 variant (e.g., encoded by H3C1–H3C12 genes) is broadly expressed, while the H3.1t variant (H3-4/HIST3H3) exhibits testis-specific expression, pointing to a specialized role in chromatin organization during spermatogenesis.
  Related references: UniProt Q16695

• The N‐terminal tail of histone H3 is subject to extensive post‐translational modifications (PTMs), including methylation, acetylation, phosphorylation, citrullination, and more. These modifications form a “histone code” that influences chromatin architecture and gene activity.
  Related references: UniProt Q16695, UniProt P68431

• Dimethylation at lysine 4 (H3K4me2) is a well‐characterized epigenetic mark associated with active and poised gene promoters, as well as enhancer regions. This rabbit monoclonal antibody was raised against a synthetic dimethylated K4 peptide and enables specific detection of H3K4me2 in various assays.

Experimental Guidance and Technical Tips

• Consider validating specificity for dimethyl-K4 by peptide competition assays or by comparing signals with antibodies directed against other H3 modifications.
• For ChIP, ChIP-seq, and CUT&Tag, optimize crosslinking, chromatin fragmentation, and antibody amount for your particular cell type or tissue.
• The predicted broad cross-reactivity (Human, Mouse, Rat, Other) suggests a conserved epitope; nevertheless, confirm reactivity in your species of interest prior to large-scale studies.
• Use protease and phosphatase inhibitors during sample extraction to preserve labile histone modifications.
• The calculated molecular weight of histone H3 is approximately 15 kDa; adjust gel percentage and transfer conditions accordingly for Western blotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For epigenetic and chromatin research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing. This DiMethyl‑Histone H3‑K4 rabbit monoclonal antibody exemplifies our commitment to supplying high‑quality reagents for advanced applications such as ChIP‑seq and CUT&Tag.