Anti-Phospho-p53 (S9) Rabbit Polyclonal Antibody for WB, IP, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 (Cellular tumor antigen p53) is a multifunctional transcription factor that induces cell cycle arrest, DNA repair, or apoptosis, acting as a critical tumor suppressor. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• Apoptosis induction by p53 is mediated through stimulation of BAX and FAS antigen expression or repression of Bcl-2, and its pro-apoptotic activity is enhanced by interaction with ASPP1 or ASPP2. Related references: PMID:12524540 PMID:17189187
• p53 negatively regulates cell division by controlling expression of a set of genes required for the process, including an inhibitor of cyclin-dependent kinases. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• p53 also regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. Related references: PMID:24051492
• Subcellular localization of p53 spans the cytoplasm, nucleus, PML bodies, endoplasmic reticulum, mitochondrial matrix, and centrosome, reflecting its diverse functional roles.
• p53 undergoes extensive post‑translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, which modulate its stability, activity, and interactions.
• Multiple p53 isoforms exist with distinct tissue‑specific expression patterns and functional properties; some isoforms can enhance or suppress the activity of full‑length p53.

Experimental Guidance and Technical Tips

• Since this antibody targets a phosphorylation site, include phosphatase‑treated controls in Western blot experiments to confirm phospho‑specificity.
• A band at approximately 44 kDa is expected; pre‑incubation of the antibody with the immunizing phosphopeptide can block signal and verify specificity.
• For immunoprecipitation, use non‑denaturing lysis buffers and consider testing with DNA damage‑induced cell lysates where p53 phosphorylation is elevated.
• In ELISA applications, the antibody may serve as a detection reagent; pairing with a total p53 capture antibody allows quantitative assessment of Ser9 phosphorylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers, laboratories, and research institutes to efficiently source high‑quality IVD raw materials. Our portfolio includes phospho‑specific and total antibodies targeting key tumor suppressors such as p53, matched antibody pairs, and bulk ancillary reagents. Whether you need validated clones for immunoassay development or support in sourcing rare‑target materials, CamelBio provides comprehensive, end‑to‑end assistance from concept to clinic.