Anti-Phospho-MAP3K5-T918 Rabbit Polyclonal Antibody for WB, ELISA - Q99683

Core Product Specifications and Parameters

Biological Background: MAP3K5 Function and Localization

• MAP3K5, also known as ASK1 or MEKK5, is a serine/threonine kinase essential for MAP kinase signal transduction.
• Acts as a crucial mediator of cellular responses to environmental changes, including differentiation, survival, and apoptosis. Required for mitochondria-dependent caspase activation in apoptosis.
• Essential for innate immune response against a wide range of pathogens, mediating signals from TNF and LPS.
• Transduces signals from oxidative stress and receptor-mediated inflammatory signals. Activates downstream MKK/JNK and p38 MAPK cascades by phosphorylating MAP2K4, MAP2K3, MAP2K6, MAP2K7, which then activate p38 MAPKs and JNKs, regulating AP-1 transcription factor.
• Located in the cytoplasm and endoplasmic reticulum.
• Abundantly expressed in heart and pancreas.
• Phosphorylation at Thr918 is a critical regulatory modification for MAP3K5 activation, and this antibody specifically recognizes phosphorylated Thr918.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and an appropriate gel percentage (e.g., 8-10%) to resolve the 155 kDa protein. Validate specificity with phosphatase-treated lysates or non-phosphorylated peptide competition.
• In ELISA, the synthetic phosphorylated peptide can serve as a coating antigen; test antibody serial dilutions to establish optimal working concentration.
• To enhance phosphorylation signal, consider inducing oxidative stress or inflammatory stimuli (e.g., TNF, LPS) in cell models prior to lysis.
• Reported cross-reactivity is for human; for other species, validate performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

This anti-Phospho-MAP3K5-T918 antibody is part of CamelBio's comprehensive portfolio for IVD raw materials. CamelBio is your one-stop sourcing bridge for diagnostic manufacturers and researchers, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate apoptosis and stress signaling research. From concept to clinic, we source rare-target raw materials to meet your assay development needs.