Anti-Phospho-Synapsin-1 (Ser9) Polyclonal Antibody for WB and ELISA - P17600

Core Product Specifications and Parameters

Biological Background: Synapsin-1 Function and Localization

• Synapsin-1 is a neuronal phosphoprotein that coats synaptic vesicles and binds to the cytoskeleton, acting as a key regulator of synaptic vesicle trafficking and neurotransmitter release at the pre-synaptic terminal. Related references: PMID:21441247 PMID:23406870
• It is involved in the regulation of axon outgrowth and synaptogenesis, contributing to neuronal development and plasticity.
• Synapsin-1 forms a complex with NOS1 and CAPON proteins, which is necessary for specific nitric-oxide functions at a presynaptic level.
• The protein is encoded by the SYN1 gene and undergoes alternative splicing, producing isoforms such as SYN1a and SYN1b.
• Synapsin-1 is localized to synaptic vesicles, the Golgi apparatus, and presynaptic terminals, with a dynamic distribution linked to its phosphorylation state.
• It is subject to multiple post-translational modifications including phosphorylation (e.g., at Ser9), methylation, and glycosylation, which modulate its activity and interactions.
• Mutations in SYN1 are associated with neurological disorders such as epilepsy and intellectual disability, highlighting its critical role in synaptic function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser9 of human Synapsin-1; consider using a non-phosphorylated peptide control to confirm phosphorylation specificity in assays.
• For Western blotting (WB), the antibody detects a band at approximately 74 kDa; validate detection in neuronal tissue lysates or synaptosome preparations.
• In ELISA applications, consider using the antibody in a sandwich format with a capture antibody targeting total Synapsin-1 to quantify phosphorylation levels.
• Cross-reactivity with mouse and rat suggests suitability for rodent models; however, validate in the relevant sample system to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including this anti-phospho-Synapsin-1 (Ser9) antibody for neuroscience and synaptic research. Our one-stop platform provides validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your assay development from concept to clinic.