Phospho-p53-S9 Rabbit mAb for WB, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 (Cellular tumor antigen p53, TP53) is a multifunctional transcription factor and key tumor suppressor that induces cell cycle arrest, DNA repair, or apoptosis in response to cellular stress. (Derived from FUNCTION: PubMed:11025664, 12524540, 12810724)
  Related references:
  PMID:11025664
  PMID:12524540
  PMID:12810724

• It negatively regulates cell division by controlling the expression of genes such as the cyclin-dependent kinase inhibitor p21, and promotes apoptosis via stimulation of BAX and FAS or repression of Bcl-2. (FUNCTION: PubMed:12524540, 17189187)
  Related references:
  PMID:12524540
  PMID:17189187

• p53 is localized in multiple subcellular compartments including the nucleus, cytoplasm, mitochondria, and endoplasmic reticulum, reflecting its diverse functional roles. It localizes to PML bodies and centrosomes, and shuttles between nucleus and cytoplasm. (SUBCELLULAR LOCATION)

• Serine 9 (S9) phosphorylation is one of the key post-translational modifications that regulates p53 stability and activity, along with acetylation, methylation, ubiquitination, and sumoylation. (KEYWORDS: Phosphoprotein, Acetylation, Methylation, Ubl conjugation)

• Multiple isoforms of p53 are generated through alternative promoter usage and splicing, with tissue-dependent expression; isoform 2 enhances transactivation activity of isoform 1, while isoforms 4 and 7 suppress it. (FUNCTION and TISSUE SPECIFICITY)

• Beyond its canonical roles, p53 participates in circadian clock regulation by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492), and is implicated in oxidative stress-induced necrosis and Notch signaling.
  Related references:
  PMID:24051492

• p53 is ubiquitously expressed, but individual isoforms show tissue-specific patterns; for example, isoform 2 is absent from brain, lung, prostate, and muscle. (TISSUE SPECIFICITY)

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Serine 9 of human p53, ensuring high specificity for the phosphorylated form. Verify phosphorylation-dependent detection by treating samples with phosphatase.
• Recommended applications include Western blot (WB) at 44 kDa and ELISA. For WB, consider using a phospho-specific blocking agent and optimized antibody dilution.
• This rabbit monoclonal antibody cross-reacts with mouse p53, making it suitable for mouse model studies. Validate in relevant tissue or cell lines.
• When using this antibody for functional studies, note that p53 activation is highly context-dependent; combine with other readouts like p21 induction or apoptosis markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for one-stop IVD raw material sourcing, offering a broad portfolio of validated antibodies, antibody pairs, and ancillary reagents to accelerate diagnostic development. The Phospho-p53-S9 Rabbit mAb supports research and assay development in oncology, apoptosis, and cell cycle regulation, a critical area for liquid biopsy and companion diagnostics. Beyond this product, CamelBio provides customized bulk supply and rare target sourcing solutions, bridging the gap from concept to clinic.