Anti-Phospho-SRF (Ser103) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P11831

Core Product Specifications and Parameters

Biological Background: SRF Function and Localization

• SRF (serum response factor) is a transcription factor encoded by the SRF gene.
• It binds the serum response element (SRE) in promoters of immediate-early genes such as FOS.
• Together with the MRTFA coactivator, SRF regulates cytoskeletal gene expression in response to Rho GTPase signaling and changes in cellular G-actin concentration.
• This pathway couples actin dynamics to transcriptional control, driving cell migration, morphogenesis, and development.
• SRF is required for cardiac differentiation and maturation.
• The protein localizes predominantly to the nucleus.
• Post-translational modifications, including phosphorylation at Ser103, modulate its activity; SRF is classified as a phosphoprotein and glycoprotein (3D-structure available).

Experimental Guidance and Technical Tips

• For Western blot, consider treating lysates with lambda phosphatase to confirm phospho-specificity and using a non-phospho peptide block as a negative control.
• IHC-P may require heat-induced antigen retrieval; validate in tissues with confirmed SRF expression, such as heart or smooth muscle.
• In ELISA, phospho-SRF can be quantified, but ensure capture and detection antibodies are optimized and include phospho/non-phospho peptide standards for calibration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop supply of IVD-grade raw materials, including validated antibodies, antigens, and ancillary reagents. This phospho-SRF (Ser103) antibody supports investigations into transcription factor signaling and cytoskeletal dynamics. For bulk orders, custom conjugations, or assay development consulting, our team offers tailored solutions to accelerate your product pipeline.