Anti-Phospho-Stathmin 1 (Ser25) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P16949

Core Product Specifications and Parameters

Biological Background: Stathmin 1 Function and Localization

• Stathmin (also known as Oncoprotein 18, Op18, metablastin, and LAP18) is a ~17 kDa cytosolic phosphoprotein that acts as a key regulator of microtubule dynamics.
• It promotes microtubule catastrophe and prevents tubulin polymerization, thereby destabilizing microtubules and influencing processes such as cell division, migration, and intracellular transport.
• Stathmin is subject to multisite phosphorylation at conserved serine residues (e.g., Ser16, Ser25, Ser38, Ser63), which inactivates its microtubule-destabilizing function and permits microtubule growth.
• Phosphorylation at Ser16 has been specifically associated with axon formation during neurogenesis (By similarity).
• Stathmin is highly expressed in the nervous system (brain, spinal cord, cerebellum) and in proliferating tissues such as thymus, bone marrow, and testis; it is overexpressed in acute leukemia cells, underscoring its role in cell proliferation and cancer.
• Subcellularly, stathmin localizes predominantly to the cytoplasm and associates with the cytoskeleton, consistent with its function in regulating microtubule networks.
• Post-translational modifications include acetylation, phosphorylation, and methylation, which fine-tune its interactions with tubulin and other partners.

Experimental Guidance and Technical Tips

• Since the immunogen is a phosphorylated peptide, it is critical to use phosphatase inhibitors during sample preparation to preserve the phospho-epitope.
• For Western blot (WB) analysis, expect a band near 17 kDa; specificity can be confirmed by phosphatase treatment or peptide blocking experiments.
• Validate the antibody in the relevant species (human, mouse, rat) as indicated by cross-reactivity data; mouse and rat stathmin share high sequence homology with human.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions as phospho-epitopes may be sensitive to harsh treatments.
• The antibody is suitable for ELISA-based assays; consider direct coating with phospho-peptide or capture formats with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated antibodies and IVD raw materials, including this anti-Phospho-Stathmin 1 (Ser25) rabbit polyclonal antibody, ideal for oncology and neurobiology research. We support your entire development pipeline with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to streamline your path from concept to clinic with reliable, one-stop procurement solutions.