Phospho-NFKB1-S907 Rabbit Polyclonal Antibody for Western Blot - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor protein, which is proteolytically processed to produce the active p50 subunit of the NF-kappa-B transcription factor complex. Related references: PMID:1423592
• The p50 subunit, together with RELA/p65, forms the heterodimeric p65-p50 complex, a transcriptional activator that binds to the kappa-B consensus sequence in target gene promoters, regulating immune and inflammatory responses. Related references: PMID:1740106 PMID:7830764
• NFKB1/p105 also functions as a cytoplasmic inhibitor by retaining NF-kappa-B dimers in an inactive state until activation signals trigger its degradation via the I-kappa-B kinase pathway.
• The protein undergoes extensive post-translational modifications, including phosphorylation at Ser907, which influences its processing and transcriptional activity.
• In resting cells, NFKB1/p105 is predominantly cytoplasmic, but upon stimulation, the p50-containing complexes translocate to the nucleus to regulate gene expression.
• Alternative splicing generates multiple isoforms; the full-length p105 precursor has a molecular weight of approximately 105 kDa, while the mature p50 subunit is about 50 kDa.
• NF-kappa-B signaling is central to cell survival, proliferation, differentiation, and apoptosis, linking NFKB1 to cancer, chronic inflammatory diseases, and immune disorders.
• NFKB1/p105 also interacts with MAP3K8 to repress MAPK signaling, highlighting its role in crosstalk between inflammatory pathways.

Experimental Guidance and Technical Tips

• For Western blot detection of phospho-NFKB1 (Ser907), include phosphatase inhibitors during cell lysis and sample preparation to ensure phosphorylation is preserved.
• Use stimulated immune or inflammatory cell lines (e.g., TNF-alpha-treated HeLa or Jurkat cells) as positive controls to confirm antibody specificity.
• Optimize primary antibody dilution (e.g., 1:500-1:2000) and incubation conditions based on signal intensity and background levels in your system.
• Pair with a pan-NFKB1 antibody to verify total protein levels and confirm phosphorylation-specific changes.
• If using for immunohistochemistry or other applications, validate the antibody in the relevant sample system as the reagent has been tested for Western blot only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic manufacturers and research laboratories in the inflammation, immunology, and oncology fields. For NFKB1-related projects, we supply a range of phospho-specific and total antibodies, enabling robust assay development from concept to clinic. Our integrated supply chain and technical consultation streamline procurement, ensuring timely access to critical reagents for your NF-kappa-B research needs.