Anti-Phospho-p53-S315 Polyclonal Antibody for WB, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• p53 is a multifunctional transcription factor and tumor suppressor, inducing cell cycle arrest, DNA repair, or apoptosis depending on cellular context. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• It plays a central role in apoptosis via stimulation of BAX and FAS expression, repression of Bcl-2, and interaction with ASPP family proteins. Related references: PMID:12524540 PMID:17189187
• Negatively regulates cell division by controlling expression of cell cycle inhibitors, and can stop cell cycle progression by inhibiting CDK7 kinase activity upon DNA damage. Related references: PMID:11025664 PMID:12524540 PMID:12810724
• Participates in oxidative stress-induced necrosis through a transcription-independent mechanism involving mitochondrial PPIF.
• Regulates the circadian clock by repressing CLOCK-BMAL1-mediated activation of PER2 transcription. Related references: PMID:24051492
• Localizes to multiple subcellular compartments, including nucleus, cytoplasm, mitochondria, and endoplasmic reticulum, reflecting its diverse functions.
• Heavily post-translationally modified: phosphorylation (including at Ser315), acetylation, methylation, and ubiquitination modulate protein stability, DNA binding, and transcriptional activity.
• Ubiquitously expressed, with several isoforms showing tissue-dependent distribution and distinct functional properties.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, include phosphatase inhibitors in lysis buffers to preserve phosphorylation at Ser315 and validate specificity using phosphatase-treated controls.
• When performing ELISA, consider using a non-phosphorylated peptide as a negative control to confirm phospho-specific binding.
• The antibody is polyclonal and has been tested in human and rat samples; cross-reactivity with other species should be experimentally verified if needed.
• Optimize antibody dilution and incubation conditions for your specific sample type and detection system to minimize background and maximize signal.

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CamelBio empowers diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. Our dedicated sourcing support simplifies access to specialized reagents like phosphorylation-specific p53 antibodies, accelerating biomarker discovery and assay development. Whether you need bulk quantities or tailored technical consultation, CamelBio is your reliable partner from concept to clinic.