Anti-Phospho-PKC mu-S910 Rabbit Polyclonal Antibody for WB, ELISA - Q15139

Core Product Specifications and Parameters

Biological Background: Serine/threonine-protein kinase D1 (PKD/PRKD1) Function and Localization

• Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, regulating MAPK8/JNK1, Ras, and NF-kB pathways, and is essential for Golgi integrity and protein trafficking. Related references: PMID:10764790, PMID:12505989, PMID:12637538
• Phosphorylates EGFR on dual threonine residues, suppressing EGF-induced MAPK8/JNK1 activation and JUN phosphorylation. Related references: PMID:10523301
• Regulates cell survival by activating IKK complex and NF-kB under oxidative stress, and mediates genotoxic-induced apoptosis through caspase-3 cleavage at Asp-378. Related references: PMID:10764790, PMID:12505989
• Involved in cell migration, differentiation, and proliferation via HDAC5/HDAC7 nuclear export, MAPK1/3 signaling, and integrin recycling; plays a role in cardiac hypertrophy, angiogenesis, and osteoblast differentiation. Related references: PMID:18332134, PMID:19211839, PMID:18509061
• In inflammatory response, phosphorylates TLR5 and NLRP3, contributing to MAPK14 activation and inflammasome assembly. Related references: PMID:17442957
• Subcellular localization includes the cytoplasm, cell membrane, and Golgi apparatus, with enrichment at the trans-Golgi network where it regulates vesicle fission and secretory trafficking.

Experimental Guidance and Technical Tips

• For Western blot, a 4–20% gradient SDS-PAGE gel is recommended to resolve the ~102 kDa protein. Use freshly prepared lysis buffer with phosphatase inhibitors to preserve the phosphorylation signal.
• The optimal antibody dilution should be empirically determined; typical starting dilutions for phospho-specific antibodies range from 1:500 to 1:1000 for WB.
• For ELISA, consider coating with the phosphorylated peptide immunogen and using the antibody for detection; pre-adsorption with non-phosphorylated peptide can confirm specificity.
• Validate the antibody in the relevant sample system; treatment with a phosphatase inhibitor like calyculin A or stimulation with phorbol esters (e.g., PMA) may enhance detection of phosphorylated PKC mu.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop platform for IVD raw materials, including validated phospho-specific antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This antibody targeting phosphorylated PKC mu supports research into cancer, cardiac hypertrophy, and inflammatory diseases, enabling the development of novel immunoassays. Contact our team for technical consultation and reliable supply solutions to accelerate your diagnostic pipeline.