Anti-MCM6 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q14566

Core Product Specifications and Parameters

Biological Background: MCM6 Function and Localization

• MCM6 is a component of the MCM2-7 complex, which functions as the replicative helicase and is essential for the licensing and initiation of eukaryotic DNA replication.
• As a core subunit of the CDC45-MCM-GINS (CMG) helicase, MCM6 participates directly in template DNA unwinding and serves as a platform for replisome assembly. Related references: PMID:16899510 PMID:32453425 PMID:34694004
• The MCM2-7 helicase ring contains six intersubunit ATPase active sites, each formed by residues from adjacent subunits; these sites contribute differentially to overall helicase activity. Related references: PMID:32453425
• MCM6 localizes to the nucleus and associates with chromatin during S phase, consistent with its role in DNA replication.
• The protein undergoes multiple post-translational modifications, including phosphorylation and acetylation, which may regulate its activity, stability, or interactions.
• The full-length MCM6 protein has a predicted molecular mass of approximately 93 kDa (UniProt: 92889 Da), consistent with the observed band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 562–821 in the C-terminal region of human MCM6; the antibody is expected to recognize a band of ~93 kDa in human, mouse, and rat cell lysates by Western blot.
• For Western blot, consider enriching nuclear fractions to improve signal, and use appropriate loading controls.
• In immunofluorescence and immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions to detect nuclear and chromatin-bound MCM6.
• The antibody is suitable for immunoprecipitation (IP) and may co-immunoprecipitate other MCM complex members; include appropriate controls.
• Validate antibody specificity in your model system using knockout or knockdown samples, and titrate for ELISA applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, empowering diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality reagents, technical services, and consulting. For developers targeting DNA replication and cell cycle regulation, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to support your entire development workflow—from concept to clinic.