Anti-MCM2 Rabbit Polyclonal Antibody for WB, ELISA - P49736

Core Product Specifications and Parameters

Biological Background: MCM2 Function and Localization

• DNA replication licensing factor MCM2 is a core component of the MCM2-7 complex, which functions as the replicative helicase for DNA replication initiation and elongation. It is also an integral part of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds DNA during replication (PubMed:32453425, PubMed:34694004, PubMed:34700328). Related references: PMID:32453425 PMID:34694004 PMID:34700328
• The MCM2-7 ring contains six ATPase active sites formed by interaction of neighboring subunits, with a critical arginine finger motif provided in trans. These sites contribute differentially to helicase activity (PubMed:32453425). Related references: PMID:32453425
• MCM2 is essential for entry into S phase and for cell division, highlighting its key role in cell cycle progression (PubMed:8175912). Related references: PMID:8175912
• In terminally differentiated hair cells of the cochlea, MCM2 contributes to development and can induce apoptosis (PubMed:26196677). Related references: PMID:26196677
• MCM2 localizes to the nucleus and is associated with chromosomes, consistent with its function in DNA replication.
• The protein is subject to post-translational modifications such as phosphorylation and acetylation, and contains zinc-finger motifs. It is associated with non-syndromic deafness as a disease variant.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 360-700 of human MCM2, which may include conserved regions; cross-reactivity with mouse is confirmed, suggesting suitability for both species in WB and ELISA.
• For Western blot, the predicted protein weight is approximately 102 kDa; consider using a gel percentage and transfer protocol optimized for proteins in this size range.
• As with any polyclonal antibody, validate specificity and performance in the relevant sample system before quantitative use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for one-stop IVD raw material sourcing, from concept to clinic. For DNA replication and cell cycle research, we supply high-quality reagents including validated polyclonal antibodies like this anti-MCM2 antibody, as well as monoclonal antibody pairs and bulk ancillary reagents. Our extensive network enables access to rare and customized raw materials to accelerate your diagnostic development.